- mappability tracks were made by cutting up the dm6 genome into "simulated reads" of 50nt in length "50mers" and mapping them back to the dm6 genome the genome. - unique reads were filtered and made into bigwig tracks. - I also made tracks where these reads are joined into sliding windows of 250bp or 1kb for fractional-mappability-tracks that may be more pleasing to the eye in some cases to show regions of low mappability rather than specific locations. - dont be confused that there are two bw tracks for each they are identical but it is because seqNdisplayR for some reason cannot accept that i have 1 mappability track when i have 2 replicate tracks of all samples. see labbook page: https://labbook.au.dk/spaces/PRAL/pages/361816987/figures+for+Cut+tag+K27me3+at+piRNA+clusters+sorted+GSCs+vs+512c